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Asserting that indiscipline will not be tolerated in the Congress, Gehlot said those expelled were indulging in anti- party activities.
Gehlot, a former chief minister of Rajasthan, said the party will strengthened further ahead of the state Assembly elections due later this year.
Vaghela, a former chief minister, had resigned from the Congress and the post of leader of the opposition in the Assembly weeks ahead of the RS elections, but not from the House.
"We have expelled them for six years as they defied the party whip and cross-voted. We will take action against them under the anti-defection law also," said Gujarat Congress chief Bharatsinh Solanki.
Bharatsinh Solanki earlier held a meeting of party MLAs in Gandhinagar and thanked them for the victory of Ahmed Patel.