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"We have decided to declare persona non grata the Brazilian ambassador until such time as constitutional order is restored in the neighboring country" Rodriguez said referring to the government of Brazilian President Michel Temer.
And Kowalik of Canada has been carrying out "nagging, constant rude and offensive interference in Venezuela's domestic affairs."
On the Twitter account of the Canadian embassy in Caracas, she said, the assembly was described as a threat to Venezuelans' ability to elect their leaders, including their next president.
Last week, Brazil urged Venezuela -- which has been sidelined from the regional trading bloc Mercosur -- to improve its human rights record. Temer said he would welcome Caracas back into Mercosur's fold when Venezuela returns to democracy.
No time frame was set for the diplomats' departure, she said.