"I stress to you once again: the Syrian army has not, did not and will not use this kind of weapons — not just against our own people, but even against the terrorists that attack our civilians with their mortar rounds," he said.
Muallem spoke at a press conference in Damascus two days after a suspected chemical attack left at least 86 people dead in the rebel-held town of Khan Sheikhun in northwestern Syria.
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"The first air raid conducted by the Syrian army was at 11:30 am on that day (Tuesday) and it attacked an arms depot belonging to Al-Nusra Front that contained chemical weapons," he said.
Al-Nusra -- now known as Fateh al-Sham Front -- was once Al-Qaeda's Syria affiliate and is the main jihadist rival of the Islamic State group.
"Al-Nusra Front and ISIS (IS) and other organisations continue to store chemical weapons in urban and residential areas," Muallem added.
The Syrian army denied on Tuesday that it had used chemical weapons against Khan Sheikhun, and Damascus ally Moscow said "toxic substances" may have been released when the army struck a "terrorist warehouse".
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