Jarrell Miller, who was denied a license by New York boxing officials over a failed test on Wednesday, has failed a second drugs test, the US media reported.
Miller recently tested positive for human growth hormone (HGH) which is on the World Anti-Doping Agency banned list.
Miller's planned June 1 fight against world heavyweight champ Anthony Joshua had already been scuppered by the first failed drug test which came to light on Tuesday.
"Salita Promotions and Greg Cohen Promotions are very disappointed in these findings and feel awful fight fans will be deprived of what would have been a great fight," Dmitriy Salita and Greg Cohen, Miller's co-promoters, told American sports broadcaster ESPN on Friday.
"It's a terrible outcome and unfortunate situation for all parties involved."
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