The referendum is set to be held today in the three provinces that make up the Kurdistan region as well as dozens of towns and villages that are disputed, claimed by both Baghdad and the country's Kurds, including the oil-rich city of Kirkuk.
The Iraqi government "requests neighboring counties and the countries of the world to deal with the Iraqi federal government exclusively (with regards to) ports and oil," read a statement from the prime minister's national security council released last night.
Barzani detailed the abuses Iraq's Kurds have faced by Iraqi forces, including killings at the hands of former leader Saddam Hussein's army that left more than 50,000 Kurds dead.
"Only through independence can we secure a future where we will not have the past atrocities," he said.
Pressure from Baghdad and the international community to call off the referendum has mounted over the past week.
"The map of Iraq is suffering attempts at division and tearing up of a united Iraq. Discrimination between Iraqi citizens on the nationalist and ethnic foundation exposes Iraq to dangers known only by God," al-Abadi said from Baghdad.
Baghdad, the United States and the United Nations have all voiced strong opposition to the vote, warning it could further destabilize the region as Iraqi and Kurdish forces continue to battle the Islamic State group.
Turkey renewed a bill on Saturday allowing the military to intervene in Iraq and Syria if faced with national security threats, a move seen as a final warning to Iraqi Kurds.
Iranian Kurdish lawmakers condemned the independence referendum in a statement Sunday and insisted that Iraq maintain its territorial integrity, reported Iran's semi- official Fars news agency.
At the Irbil press conference, Barzani said he was unaware that Iran had closed its airspace, but that it was Iran's "own decision." The leader also confirmed that there had been shelling along Iran's border with the Kurdish region.
"There will be no benefit for either side," he said of potential border closures.
Despite fears in disputed territories Iraqi territory claimed by both the Kurds and Baghdad Barzani said he didn't expect violence to follow the vote, explaining that Iraq's military and the Kurdish fighters known as the peshmerga have "good coordination in the war against terror."
The peshmerga forces have been instructed not to respond to "provocations," in Kirkuk, Barzani added.
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