"Some activists decided to protest this US practice but it seems that they picked the wrong target," a specialised blog of the Brazilian news portal Uol said.
"They hacked NASA's web page and left the message: Stop spying on us," it said.
The hackers' message also called on the United States not to attack Syria.
A NASA spokesman confirmed that a Brazilian hacker group last week posted a political message on a number of NASA websites.
"We are diligently taking action to investigate and reconstitute the websites impacted during web defacement incident," he said.
The attack followed recent disclosures that the NSA spied on Brazilian President Dilma Rousseff's email communications and on the state-run energy giant Petrobras.
The disclosures were based on documents obtained by former US intelligence contractor Edward Snowden.
Brasilia slammed the alleged spying as "unacceptable" and demanded explanations from Washington.
Rousseff, who spoke by telephone with US President Barack Obama about the affair late yesterday, was expected to announce today whether she will go ahead with a state visit to Washington that had been planned for October 23.
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