In a letter sent by Foreign Minister Alain Aime Nyamitwe and seen by AFP, the Burundian government said the three UN investigators were declared persona non grata with immediate effect.
Pablo de Greiff from Colombia, Christof Heyns from South Africa and Maya Sahli-Fadel of Algeria had been appointed in December to lead the independent probe.
UN spokesman Stephane Dujarric expressed disapproval of Burundi's decision.
"It's critical that Burundi and every other country cooperate fully with UN human rights mechanism and that is including working with those representing it," he said.
The three experts had concluded in a report released last month that "gross human rights violations have and are taking place, committed primarily by state agents and those linked to them.
"Thousands of people have been tortured, suffered sexual abuse or disappeared, while arbitrary detention has happened "on a massive scale", the report said.
UN diplomats said the move was clearly an angry response from Bujumbura to the report which paved the way to a UN decision to set up a formal Commission of Inquiry.
Last week, a representative of the non-governmental organization Trial International was stripped of her visa and ordered to leave Burundi.
The employee was scheduled to provide legal training to Burundian lawyers defending victims of state-sponsored violence.
Burundi has been in turmoil since President Pierre Nkurunziza announced plans in April last year to run for a third term, which he went on to win.
More than 500 people have died, many of them in extrajudicial killings blamed on Burundian police, security forces and militias linked to the ruling party, according to the United Nations.
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