The incident occurred in the Beit Ummar area near the flashpoint West Bank city of Hebron as border police were conducting an operation, police said. Officers then shot the Palestinian.
A wave of Palestinian knife, gun and car-ramming attacks began a year ago, but the violence has signficantly declined in recent months.
Since October last year, the violence has claimed the lives of 236 Palestinians, 36 Israelis, two Americans, a Jordanian, an Eritrean and a Sudanese, according to an AFP count.
Human rights groups have accused Israeli security forces of using excessive and often lethal force in certain cases.
Internal reviews by the army of two fatal shootings of attackers earlier this month found that the use of deadly force could have been avoided, public radio reported last week.
Many analysts say Palestinian frustration with the Israeli occupation and settlement-building in the West Bank, comatose peace efforts and their own fractured leadership have helped feed the unrest.
Most of the attacks have been carried out by lone-wolf assailants, many of them young.
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