The pastor of the St Peter's Missionary Baptist Church died as result of injuries sustained in the shooting, which took place after 12:30 pm (17:30 GMT), WHIO television said, citing police.
A witness reported hearing two gunshots after the shooter walked into the pastor's office, the station said.
Witnesses said police later escorted a man from the church in handcuffs and put him in the back of a police cruiser, the station said.
Another television station, WDTN, cited witnesses as saying that the pastor had walked toward the pulpit inside the church when his brother, who was seated in a pew, stood up and followed him.
More than 20 people were reported to be inside the church at the time of the shooting, WDTN reported.
Police could not immediately be reached for comment.
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