Reading Buses' "Bus Hound" was recorded doing a lap speed of 76.785mph (123.57km/h) at Bedford's Millbrook Proving Ground.
It runs on biomethane compressed natural gas and is painted black and white like a Friesian cow. It normally carries passengers around Reading.
The UK Timing Association confirmed the new record, the BBC reported today.
Trevor Duckworth, the association's chief timekeeper, said this was the first time a bus had been on Millbrook Proving Ground and described it as "quite a sight".
Martijn Gilbert, chief executive of Reading Buses, said it would not be recognised as a Guinness World Record unless it reached speeds above 150mph (241km/h).
Chief engineer John Bickerton said the company wanted the "world's first service bus speed record" to bring to light the viability, power and credibility of buses fuelled by cow poo.
"We've laid down a challenge for other bus operators to best our record and we had to make it a bit hard for them.
"Most importantly we wanted to get the image of bus transport away from being dirty, smelly, and slow. We're modern, fast, and at the cutting edge of innovation.
Its fuel is made from animal waste which is broken down in a process called anaerobic digestion to produce biogas, which is then liquefied, Gilbert said.
It is stored in seven tanks fixed inside the roof of the bus.
The vehicle's name was inspired by the British Bloodhound super-sonic car which aims to go beyond 1,000mph in 2016.
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