They were published in the EU's Official Journal late in the day, meaning that they will come into effect on Friday.
The new measures, finally agreed earlier this week after months of hesitation, target Russia's banking, defence and energy sectors in view of its "actions destabilising the situation in eastern Ukraine," a statement said.
A first step limits access by Russian state-owned banks to Europe's financial markets, chief among them London, which will increase their cost of doing business and hinder their contribution to the economy.
EU nationals and companies will no longer be allowed to buy or sell new bonds, stocks or other debt instruments with a maturity of more than 90 days issued by such banks, the statement said.
Sales of arms and dual-use technology are banned, along with sensitive technologies in the oil sector, but Europe has baulked at applying sanctions to Russia's gas industry, which supplies around a third of the EU's needs.
Analysts say the oil sector ban could cause significant problems for Russia over time because it relies heavily on advanced Western drilling technology, especially for the development of shale gas reserves and new fields in extreme environments such as the Arctic.
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