The incident took place around 6.30 am after the driver of the car lost control over the vehicle and it dashed against the tree, said police.
The car was on way to Shirdi from Surat, an official at Mhasrul police station said.
The deceased were identified as Manoj Jangid (57), Ramavtar Surajsingh Kumavat (52), Santoshidevi Jangid (51), Manohar Jangid (57), Buddhi alias Gudiya Kumavat (15), all residents of Dujod in Rajasthan.
One of the injured Santoshi Kumavat is being treated at Nashik civil hospital, police added.
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