Rane has forwarded his resignation to the chief minister Prithviraj Chavan, sources said.
Nilesh lost to Shiv Sena nominee Vinayak Raut in the close contest.
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"Although Rane has tendered his resignation over the loss of his son Nilesh, the same will not be accepted. We will hold discussions with him and find a way out," Thakre told PTI.
Meanwhile, Shiv Sena president Uddhav Thakeray has left for Ratnagiri district where he is likely to address a 'victory rally' for Raut.
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