Noor Salman, 30, entered her plea in an Oakland, California, courtroom on yesterday, two days after she was taken into custody at the home she shared with her mother in suburban San Francisco.
Her arrest came after she was interviewed numerous times by FBI agents investigating the June 12 attack in Florida.
Federal prosecutor Roger Handberg said in court Tuesday that Salman knew about the plan by her husband Omar Mateen to attack the gay nightclub and then lied to investigators after it was over. Mateen was killed at the scene by authorities.
Salman will return to court on February 1 to argue for her release pending trial on the counts that could result in a life sentence if she is convicted.
Salman's uncle Al Salman has defended his niece, saying she is an innocent person who was physically and mentally abused by Mateen.
He said she remained in the marriage because she feared losing custody of the couple's 4-year-old boy.
Noor Salman was living with Mateen in Fort Pierce, Florida when he proclaimed his allegiance to Islamic State and attacked the nightclub.
Charles Swift, her lead attorney, declined comment yesterday outside court. He is director of the Texas-based Constitutional Law Center for Muslims in America,
Salman told The New York Times in an interview published in November that she knew her husband had watched jihadist videos but that she was "unaware of everything" regarding his intent to shoot up the club. Salman also said he had physically abused her.
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