World number three Roger Federer pulled out of the Italian Open before his quarter-final match on Friday with a right leg injury.
Federer, a four-time Rome finalist, had been due to meet Greek eighth seed Stefanos Tsitsipas for a place in the semi-finals.
The Swiss 20-time Grand Slam winner was playing in Rome to prepare for his return to the French Open for the first time since 2015.
"I am disappointed that I will not be able to compete today," said Federer.
"I am not 100 percent physically and after consultation with my team, it was determined that I not play.
"Rome has always been one of my favourite cities to visit and I hope to be back next year."
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