The fifth seed Indo-American pair lost 5-7 1-6 to the unseeded Russian-Czech pair in one hour and 10 minutes in the second round of the euro 4,033,254 clay court event.
Sania and Bethanie could convert just two of the eighth break chances they had against their rivals while dropped their serve on six occasions.
Sania and her partner shared 12,000 euro as prize money and earned 140 ranking points each.
The Roland Garros is slated to begin from May 26.
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