Irfan, who represented Bilari Assembly seat, was on his way to attend the marriage of son of senior cabinet minister Shivpal Singh Yadav in Saifai in Etawah district when his vehicle overturned and fell into a ditch on Thursday.
The legislator later succumbed to his injuries while SP leader KP Singh and driver Farzad Ali were killed on the spot, police had said.
District Magistrate Shambhunath, who conducted a preliminary inquiry into alleged lapses, directed ADM Ashok Kumar Srivastava to submit his probe report within 15 days.
Shambhunath said he received complaints that the MLA was administered "injections containing steroid, which led to internal bleeding".
He said the cylinder present in the ambulance allegedly did not have oxygen and life saving drugs were missing.
The DM said the inquiry against the hospital administration will cover 30 points.
Meanwhile, opposition BJP attacked Uttar Pradesh Chief Minister Akhilesh Yadav, saying his "tall claims" of better health and ambulance services in the state have been exposed by the incident.
