Myanmar's de facto leader received the award at the Guildhall, after meeting with Queen Elizabeth II and heir to the throne Prince Charles on Friday.
The award was in recognition of her "non-violent struggle over many years for democracy and her steadfast dedication to create a society where people can live in peace, security and freedom", the City of London said.
Nobel Peace Prize laureate Suu Kyi is on a tour around Europe that has already taken her to Belgium, Italy and the Vatican City.
They chanted "Suu Kyi is lying; Rohingyas are dying" and said that people who had once supported her now opposed her.
"We believed that she would be a champion of human rights but she has been complicit with... The junta murdering innocent Rohingyas," one speaker said.
Last week Suu Kyi rejected a decision by the UN's rights council to investigate allegations of crimes by Myanmar's security forces against Rohingyas.
Myanmar is scheduled hold peace talks this month aimed at ending decades-long ethnic wars that have intensified since Suu Kyi's party took power a year ago.
She also met European Council president Donald Tusk and European Commission foreign policy chief Federica Mogherini while in Brussels.
She then met Pope Francis and Italian Prime Minister Paolo Gentiloni while in Rome on Thursday.
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