Bolt, the 100m world record-holder who turns 30 on the final day of the Rio Games, won his semi-final heat in 10.04 yesterday despite a slow start then left the National Stadium well before what was to have been a showcase 100m final.
"After feeling discomfort in my hamstring after the first round last night and then again in the semi-final tonight, I was examined by the Chief Doctor of the National Championships and diagnosed with a Grade 1 tear," Bolt said in a statement posted on his Twitter site.
Missing the meet does not end Bolt's dream of trying to win his third consecutive Olympic gold medals in the 100, 200 and 4x100 next month in Brazil.
The Jamaican athletics governing body allows for medical exemptions if an athlete can show an injury denied a chance to compete at the trials. But Bolt must also prove his fitness and that makes his planned run in three weeks at a Diamond League meet in London crucial to proving his case.
Former world champion Yohan Blake won his 100m semi-final heat in 9.95 seconds with Nickel Ashmeade second in 10.01 and Asafa Powell third in 10.02.
Bolt, who set the 100 world record at 9.58, edged Senoj-Jay Givans on the line by .01 of a second.
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