Davutoglu was speaking to reporters in the central Turkish city of Konya, a day after meeting with exiled Hamas chief Khaled Mashaal.
Violent demonstrations led Israel in November to briefly restrict Muslim access to a holy site in Jerusalem that includes the Al-Aqsa Mosque, Islam's third most sacred place, and the ancient Hebrew Temple Mount, the holiest place in Judaism.
The move fueled accusations that Israel was secretly plotting to take over the site, despite Israeli denials. Davutoglu said: "Turkey won't accept any impositions concerning Al-Aqsa."
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