The jihadists also reportedly killed another 40 former Iraqi Security Force (ISF) members before dumping their bodies in the river, UN rights office spokeswoman Ravina Shamdasani told reporters in Geneva.
The rights office has listed numerous IS atrocities, including tens of thousands of forced relocations and hundreds of executions, allegedly committed in and around Mosul since a government operation to retake the northern city began last month.
Some of the allegations have been termed "preliminary" and needing more investigation.
In the early hours of yesterday, IS fighters "brought dozens of long trucks and mini-buses to Hamam al-Alil City, south of Mosul, in an attempt to forcibly transfer some 25,000 civilians towards locations in and around Mosul," the rights office said in a statement.
Most of the vehicles were prevented from reaching Mosul because of coalition aircraft patrolling the area, the statement added.
Shamdasani said there was "a pattern" of the jihadists surrounding their offices and bases with civilians.
Separately on Saturday, 40 ex-ISF officers "were killed and their bodies thrown in the Tigris River," after being kidnapped by IS earlier in the week, Shamdasani further said.
That brings to 296 the number of former Iraqi security officers killed by IS since last Tuesday, according to the UN.
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