"Just setting up my twttr...Again (account suspension was an internal mistake)," Dorsey tweeted late yesterday.
For about 15 minutes those trying to access Dorsey's account received a message stating that the account was suspended.
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The company did not respond to an AFP inquiry.
The incident came as Twitter is struggling to increase its user base and seeking a path to profitability.
Co-founder Dorsey returned as chief executive last year but has yet to ignite growth.
Just weeks after reporting job cuts and a disappointing fiscal quarter, Twitter announced earlier this month that chief operating officer Adam Bain was leaving the company.
Twitter was widely reported to be in talks to find a buyer but no deal has materialized.
The company recently said it would cut nine percent of its workforce and discontinue its looping video application Vine in the coming months.
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