The 35-year-old actor was pulled over at 1:22am in his Dodge Challenger, initially because of his tinted windows, according to a NYPD public information officer, reported E1 online.
But on further inspection, the officers detected alcohol on Mackie's breath and spotted his bloodshot eyes.
After Mackie failed field sobriety tests, he was arrested and charged with driving while intoxicated or DWI.
The actor's license was also suspended because he refused to take a blood-alcohol test.
Mackie is known for his roles in '8 Mile', 'Gangster Squad' and most recently opposite Ben Affleck and Justin Timberlake in 'Runner Runner'. He will next be seen in 'Captain America: The Winter Soldier'.
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