MPs and leaders from around 10 political parties including Samajwadi Party, Trinamool Congress, CPI and CPI(M) participated in the protest march amid heavy security.
The Congress and other Opposition parties have been opposing the Land Acquisition Bill terming it as anti-farmer and pro-corporate.
The protesters shouted anti-government slogans resolving to continue their opposition till the Modi government makes changes in the Bill.
A large contingent of security personnel drawn from the Delhi Police and paramilitary forces was deployed in the area in riot gear.
The Lok Sabha had cleared the Bill last week and the government now faces its real test in the Rajya Sabha where it is in a minority.
