Members of 11 trade unions, including, CITU, INTUC and DMK's Labour Progressive Front, are participating in the strike, which caught the holiday commuters by surprise as it was advanced by a day.
About 60 per cent of buses did not operate following the strike, causing immense hardship to travellers, police and trade union sources said.
In Chennai, suburban trains saw rush of passengers in view of the bus strike while autorickshaws and share autos were in demand.
Various political parties, including DMK, PMK and Congress urged the Government to convene conciliatory talks immediately with the striking unions and arrive at an amicable settlement.
CITU state general secretary, A Sounderrajan said the Unions would continue their strike till the Government concede to their demands, including wage revision.
The striking employees held dharnas at Tamil Nadu State Express Corporation office here and at various other places across the state shouting slogans demanding the authorities to take urgent steps to resolve their grievances.
Allaying concerns about the strike's impact tomorrow, being the first working day of the week, various District Collectors announced that bus services would be operated as usual.
In Tirunelveli, two passengers were injured when unidentified miscreants pelted stones at as many buses, police said, adding they had been hospitalised.
Out of the around 1,500 buses in Tuticorin and Tirunelveli districts, only around 700 were operated today, officials said.
District Collector M Karunakaran said buses would ply as usual from tomorrow.
A report from Erode said only ten per cent the buses were operated in the district.
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