Sumbal Bibi sustained bullet injuries when the vaccination team was attacked by suspected militants at Badabher, a suburb of Peshawar, on May 28.
She succumbed to her injuries in Lady Reading Hospital.
Sharafat Bibi, another health worker, was killed instantly during the attack.
The team was attacked while the health workers were going from door to door to vaccinate children against the crippling disease.
The attack had prompted the World Health Organisation to suspend its vaccination campaign in Peshawar.
Pakistan is one of a handful of countries where polio is still endemic.
