Some mediapersons were also injured during the protest.
After registering a case of rioting and damage to public property against the protesting students, police have arrested 52 students out of the 66 identified by the university authorities, police said.
The agitating students wanted to hold talks with Vice Chancellor Arun Kumar Grover on the fee hike issue as they were observing "PU Bandh" against the recent hike.
Police resorted to water cannons to disperse the angry students. The situation turned violent when students allegedly started pelting stones and flower pots at cops, forcing the police to lathi charge and lob teargas shells, leading to injuries to several students including girls.
Several student bodies including Students for Society (SFS), National Students Union of India (NSUI), Punjab University Students Union (PUSU) had given a joint call for 'Panjab University Bandh' in protest against the fee hike.
"On our side 22 cops sustained injuries," SP said.
"We have arrested 52 students so far for rioting and damaging public property," he said.
After police used force, some protesting students took shelter in a Gurdwara situated on the campus.
Earlier, the protesting students boycotted classes.
Some students could also be seen forcing the shops to close.
Some photo journalists and reporters who were covering the protest have also sustained injuries allegedly caused by police cane charge.
In a release issued here, the Press Club's president Jaswant Rana and Secretary General Barinder Singh Rawat alongwith the governing council members demanded inquiry into the incident and registration of FIR against those police personal who were involved in lathicharge.
A delegation of the Chandigarh Press Club will meet the UT Administrator, UT Adviser and UT IGP in this regard to take strict action against those involved in this incident, the release said.
The students have been protesting against the "significant" hike in tuition fee announced by the PU senate last month for the 2017-18 academic year.
With the students demanding rollback of the fee hike, the PU had justified the average 12.5 per cent hike while considering the financial condition of the university as the it has anticipated a deficit of Rs 244 crore for the financial year 2017-18.
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