The two stars, who last worked together in "Raavan", are appearing in a movie together after a gap of five years.
Director Sanjay Gupta shared the news with fans on his Twitter account.
"Big excitement at the shoot today as Abhishek Bachchan shoots his special appearance in JAZBAA," Gupta tweeted.
They earlier worked together in "Guru", "Dhoom 2", "Kuch Na Kaho" and "Umrao Jaan", among others.
"Jazbaa", also stars Irrfan Khan and will be Aishwarya's first movie after the birth of her daughter Aradhaya in 2011.
For the film is October 9.
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