Prasanna "aggravated" his back injury after completing 50 when he collided with Bengal pacer Ashok Dinda while running from the non-striking end. He had to be stretchered off the field.
The captain could not take the field again and Tamil Nadu's innings folded for 246/9 in 88.4 overs. The skipper said he is doubtful to take field again in the match.
The collision happened in the last ball of 84th over when Dinda tried to cross the pitch to collect the ball at the mid-on region and collided with Prasanna who was responding to Mohammed's call for a single.
After managing to complete the run Prasanna lay on the pitch and was soon stretchered off the field before being rushed to the hospital.
Nothing went right for Tamil Nadu as they would lament dropping a regulation catch of Tiwary at second slip by K Bharath Shankar.
Barring the blip, the Bengal batsman was all elegance and looked untroubled in his 81-run partnership with Easwaran.
Bengal started out with a positive manner with openers Arindam Das and Rohan Banerjee putting on the foundation with a 74-run partnership.
Debutant left-arm quick T Natarjan got his maiden first class scalp by dismissing Banerjee who got a thick edge to second slip to be out for 27 from 83 balls.
But Tiwary looked unperturbed and played strokes all around the wicket cruising to his 18th first class half-century in 59 balls as the visitors looked to be running out of ideas.
Brief Scores
Tamil Nadu 246/9; 88.4 overs (R Prasanna 50 retired hurt; Ashok Dinda 4/76).
Bengal 183/2; 52 overs (Manoj Tiwary 54 batting, Abhimanyu Easwaran 41 batting, Arindam Das 48). Match to continue.
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