He was 81 and was keeping unwell for the past few months. He is survived by his wife and a daughter.
From the fifties till the eighties, Sen had sung a number of hit songs. He had also sung duets with Asha Bhonsle and Lata Mangeshkar.
Some of his well-known songs include 'Aa ja re nain dware' from the Dev Anand-Waheeda Rehman starrer 'Roop Ki Rani Choron Ka Raja' (1961) and 'Main rangeela pyar ka raahi' in 'Chhoti Bahan'.
West Bengal Chief Minister Mamata Banerjee condoled Sen's death.
"Saddened at the passing away of legendary singer Subir Sen. He is our Banga Bibhushan. He will always be in our hearts," Banerjee tweeted.
