European Parliament lawmakers and staff gathered outside the building in the rain in Brussels at 10:45 am (0945 GMT) to "show their solidarity" with France, president Martin Schulz said.
Separately European Commissioners observed a minute's silence with the Latvian government at a meeting in Riga to mark the start of Latvia's six-month presidency of the European Union.
The European Commission, the executive arm of the 28-nation EU, also said all activity at its offices in central Brussels would also come to a halt at midday (1100 GMT) as a mark of respect.
Meanwhile the blue EU flag with yellow stars is also being flown at half-mast on EU buildings to mark the killings.
The attack has stoked fears of other attacks as Jihadi fighters return radicalised to Europe from the bloody conflicts in Syria and Iraq.
In France, police are seeking two brothers in connection with yesterday's attack, one of them Cherif Kouachi, being a jihadist who has been well-known to anti-terror police for many years.
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