Prosecutors are investigating the cause of the blaze early today in the Centocelle neighbourhood.
The ANSA news agency said the sisters were aged 4, 8 and 20, and were Gypsies, also known as Roma. It said other family members managed to escape.
Rome police Cmdr Mario De Sclavis said the victims weren't well known to area residents, who reported hearing a huge explosion followed by screams.
Mayor Virginia Raggi visited the site of the blaze today and asked for a moment of silence.
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