Israel and its perceived supporters in the United States and Britain are still the main bogeymen of the Quds (Jerusalem) Day protests, which saw thousands more rally across Iran and in allied countries.
But the focus has also turned to the deepening rivalry with Saudi Arabia and jihadists such as the Islamic State (IS) group.
Sajad Saber, a 24-year-old student, said it was the first time he had attended the rally in Tehran - triggered by the death of a friend who had gone to fight in Syria.
Iran is fighting IS and other jihadist groups in Syria as part of its support for the regime of Bashar al-Assad.
Huge groups of protesters converged on Tehran University under a blistering summer sun.
Many - from young children to elderly ladies - sported red bandanas with "I love fighting Israel" written on them.
President Hassan Rouhani, attending the rally, said regional conflicts were making it increasingly difficult to address the Palestinian issue.
"Today, due to the divisions in the Islamic world, the presence of takfiris (Sunni extremists) and terrorists in the region... We are far away from the ideal of liberating Palestine," he told state TV, calling for greater unity between Muslims.
"Daesh is the creation of Israel. They want Muslims to fight each other and Palestine to be forgotten," said Ali Kotobi, a 63-year-old Iranian born in Iraq, who said his cousins were currently fighting IS across the border.
However, much of the vitriol at the protests was also turned on Iran's main regional rival, Saudi Arabia.
Tensions between the two powers have plummeted over their support for opposing sides in wars in Yemen and Syria.
They have been further soured by the failure to agree on terms for Iranian pilgrims to visit Saudi Arabia for the annual hajj in September.
One group carried a fake coffin featuring Saudi Arabia's leaders and slogans against Daesh Sunni extremists.
Opposition to Israel and support for the Palestinian cause has been a central issue for the Islamic republic since it took power in 1979.
Quds Day was inaugurated by the late revolutionary leader Ayatollah Ruhollah Khomeini, and has become a popular way for the Iranian government to rally the faithful.
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