Predicting the demise of "rabid dog" Israel, which Iran has accused of trying to "torpedo" a deal, Ayatollah Ali Khamenei said the powers must respect the Islamic republic's "red lines".
"I insist on not retreating one step from the rights of the Iranian nation," Khamenei, 74, told militiamen of the Basij force in a rare, live televised address.
France said that his comments -- he said Israel's leaders were "not worthy to be called human" -- are "unacceptable and complicate negotiations" on Iran's nuclear programme.
But Israel, widely assumed to have a formidable nuclear arsenal itself although it has never admitted it, has expressed alarm at the mooted deal on the table in Geneva.
Instead of stopping all uranium enrichment, as multiple UN Security Council resolutions have demanded, the powers appear to be happy with a suspension of enrichment at medium levels.
Along with other steps, this would be a "first-phase" deal while a long-term accord is hammered out by Iran and the United States, China, Russia, Britain, France and Germany, known collectively as the P5+1.
"You are not really dismantling any capacity to make fissile material for nuclear weapons," he said in an interview.
Israel has refused to rule out bombing Iran, as it was assumed to do with an Iraqi reactor in 1981 and a Syrian site in 2007. Iran says its programme is peaceful and the West should condemn Israel's nuclear arsenal.
Uranium enrichment is the main worry for the international community since enriched uranium has civilian uses but also can go into a bomb.
It remains to be seen whether Iran, seeking an easing of UN, US and EU sanctions that have more than halved the country's lifeblood oil exports, will accept what it is being offered in return.
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