"The ambassador has been declared persona non grata," Malaysia's foreign ministry said in a statement, adding it had demanded but not received an apology over Pyongyang's attack on its investigation of the case.
"The expulsion of the DPRK (North Korea) Ambassador is... an indication of the government's concern that Malaysia may have been used for illegal activities," the statement added.
Kim Jong-Nam, 45, was poisoned last month with VX, a nerve agent so deadly that it is classed as a weapon of mass destruction.
South Korea has blamed the North for the murder, citing what they say was a standing order from leader Kim Jong-Un to kill his exiled half-brother who may have been seen as a potential rival.
North Korea, which has not acknowledged the dead man's identity, has vehemently protested the investigation, saying Malaysia is in cahoots with its enemies.
In response, Malaysia has cancelled a rare visa-free travel deal with North Korea -- a key conduit to the outside world -- and recalled its envoy to Pyongyang, putting the skids under once cozy ties.
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