The song, "The Wolf", highlights the band's much-talked-about electric banjo-less new sound as well as band leader Marcus Mumford's vocals, reported Aceshowbiz.
Unlike its predecessor, the mellow lead single "Believe", "The Wolf" is a more straightforward rock tune.
"Wilder Mind" comes to follow up the group's critically-acclaimed 2012 effort "Babel" which won the coveted Album of the Year at the 2013 Grammy Awards.
It is set to hit the stores on May 4.