Nearly 200 protesters blocked National Highway-3, which links Agra with Mumbai, near Pigdambar crossing in Kishanganj area in Indore district and broke glass bottles on the road, police said.
Heated arguments ensued between the protesters, who included members of Karni Sena, and the police when the Rajput group members tried to stop vehicles as part of their protest.
Talking to PTI, Additional Superintendent of Police Nagendra Singh denied reports of stone-pelting during the clashes. He said the police managed to bring the situation under control.
Around 50 Karni Sena activists armed with sticks burnt an effigy of Bhansali, the film's director, on Indore-Depalpur road, police said. A police officer said the road was cleared after the protesters were given a warning.
Agitations were also carried out in Sagar, Guna, Bhopal, Ratlam and other towns in Madhya Pradesh against the movie.
The film, which was earlier named "Padmavati" and was slated for a December 1 release, will now hit theatres nationwide tomorrow.
The film has been been facing protests by Karni Sena and other fringe groups over allegations that it distorts historical facts.
The film's producers have denied the allegations.
The Supreme Court last week paved the way for its nationwide release by staying the notifications and orders issued by Madhya Pradesh, Rajasthan and Gujarat - all ruled by the BJP - prohibiting exhibition of the film in the states.
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