A plunge in rupee value also hit investor sentiment. Traders also said weak global cues due to poor Chinese manufacturing data put pressure on markets.
The 30-share barometer resumed weak and was in the negative territory for the entire day.
The key index settled at 21,133.56, a fall of 240.10 points or 1.12 per cent, recording the biggest drop in more than three weeks. On January 2, it declined 252 points.
In the previous 4 days, the bellwether index had gained 310 points to set new closing peaks on rate cut hopes after wholesale and retail inflation softened. The barometer rose by 87 points to close at all-time high of 21,337.67 yesterday.
Brokers said Rajan's comment that inflation was a "destructive disease" killed rate cut hopes. Rupee weakness also added to worries of investors who pressed the selling button, they added.
SBI, ICICI Bank and HDFC lost 2.12 per cent, 1.96 per cent and 1.31 per cent, respectively. Martui Suzuki (1.25 per cent), Cipla (1.08 per cent) and Sun Pharma (1 per cent) were other big losers.
All the 12 sectoral indices closed with losses in the range of 0.28 per cent and 3.19 per cent. Interest rate sensitive realty, banking and auto ended lower by 3.19 per cent, banking 1.88 per cent and auto 1.57 per cent in that order.
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