Vladimir Semago, who hosted "In My Own Words" on Capital Radio, said he had been fired last week after hosting a live broadcast in the Russian capital on the day Crimea voted to sever its ties to Ukraine and join Russia.
Semago told Echo of Moscow radio that he had tried to "talk to people and find out whether they are really all so united" behind Russia's move to absorb the breakaway peninsula.
"For that, I received yet another rebuke, an accusation of being unethical and acting provocatively," he said.
The editor-in-chief of Capital Radio, a talk-radio station that broadcasts in Moscow and the surrounding region, questioned Semago's version of events.
"It's very fashionable to use a political position on Crimea as an explanation now. You just need to work professionally, that's all," Roman Babayan told Echo of Moscow.
He said the station would release a statement tomorrow.
Semago said he did not oppose Crimea becoming part of Russia but was uneasy with the way the annexation was implemented.
Semago, a businessman and former lawmaker, had worked at the radio station for two years.
He served as a Communist lawmaker in the 1990s before being expelled from the party and later serving for a term as an MP for the ruling United Russia party.
There are fewer and fewer outlets in the Russian media for political views in opposition to the Kremlin.