However, smallcap and midcap stocks outshone bluechips.
The BSE Sensex, which had gained 392.41 in past two days, started on a positive note tracking overnight gains in US markets but fell by 68.32 points, or 0.32 per cent, to end at 21,032.71 dragged down by HDFC Bank, HDFC and Sun Pharma.
Sesa Sterlite fell over 2 per cent after CBI registered a preliminary enquiry against Vedanta Group chief Anil Agarwal in connection with alleged irregularities in Hindustan Zinc disinvestment.
The 50-share NSE Nifty slid 16.10 points, or 0.26 per cent, to end at 6,248.40 hurt by losses in metal and banking scrips. SX40 index of MCX-SX fell 49.05 points to 12,510.16.
"The rally in the US markets which are breaching 6-year high levels did not culminate fully into the Indian markets. The session on Thursday, the last for December series and for the calendar year may turn out to be volatile as we head for the Q3 results season," said Vishal Jajoo, Senior Research Analyst (PCG), Nirmal Bang Securities.
Yesterday, key US indices -- Dow Jones Industrial Average and S&P 500 -- carried on their record run in the holiday shortened week as incoming economic data reinforced perception that economic recovery will accelerate in the year ahead.
In broader markets, GMR rose after reports said Malaysia Airports Holdings Berhad plans to buy the entire 40 per cent stake of the Indian firm in Istanbul's Sabiha Gokcen International Airport project for 225 million euro.
Apollo Tyres surged 8 per cent on hopes the company will get a better deal to acquire Cooper Tire & Rubber Co.
The BSE Metal index fell the most by losing 1.09 per cent, followed by Banking index (0.43 pc), Oil and Gas index (0.23 pc), Power index (0.22 pc).
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