The UN has described the appeal as the largest ever in its history for a single humanitarian emergency.
UN Secretary General Ban Ki-Moon will chair the one-day ministerial-level Second International Pledging Conference for Syria, which will be opened by Kuwait's Emir Sheikh Sabah al-Ahmad al-Sabah.
UN Secretary General and Regional Humanitarian Coordinator Nigel Fisher has told Kuwait's official KUNA news agency that the funds will be used to aid some 13.4 million Syrians whom the United Nations estimates will be affected by the Syrian civil war by the end of 2014.
The United Nations will need USD 2.3 billion to support 9.3 million people inside Syria and USD 4.2 billion for Syrian refugees, expected to nearly double to 4.1 million by year's end, the UN official said.
The World Food Programme said it was stepping up food aid and would also provide supplements to around 240,000 toddlers aged 6-23 months, to ensure they do not suffer from malnutrition.
"This is the worst humanitarian crisis that we have seen in decades, with every day more vulnerable Syrians pushed into hunger," said WFP Syria emergency coordinator Muhannad Hadi last month.
The Swiss talks have been organised in an attempt to revive a long-stalled framework for peace involving a cessation of hostilities and the creation of a national transitional government that could involve figures from the current regime and the opposition.
Ahead of his arrival in Kuwait City, Ban warned that the humanitarian situation in Syria has been deteriorating and called on donor nations to help meet the target.
The situation has reached a "very serious... And critical" level, Ban said in an interview with KUNA.
"I sincerely hope that member states will come (to the Kuwait Conference) with generous helping hands" for the Syrian people inside and outside the country, he said.
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