Pictures and video showing the tiger roaming among cars on the Doha Expressway flooded social media in the tiny Gulf country.
Footage including a 20-second video on YouTube and Twitter showed the big cat running through lanes of heavy traffic, apparently trying to find a way off the congested road.
Footage also emerged showing the tiger initially falling onto the road from a moving truck.
The interior ministry took to Twitter to say it was investigating the incident.
Twitter users said the incident happened today morning, although this could not be immediately verified.
One picture released on social media claimed to show that the big cat had been captured safe and sound.
It was not clear who owns the tiger, but keeping wild animals, including tigers, as pets is not uncommon in wealthy Gulf countries.
Last year, the government had to make an appeal for the owners of an escaped cheetah cub to come forward after it was found north of Doha.
One social media user, Sultan Al-Qassemi, complained on Twitter after seeing the footage that: "This obsession of keeping wild animals by rich kids in the Gulf has gone too far.
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