Red Crescent official Ammar Lamloum told AFP that the group of migrants, mainly Africans, had been trying to reach the Italian island of Lampedusa when they were rescued.
"They are now at the port in Ben Guerdane," he said.
Tunisian and Italian authorities have been at the forefront of rescue efforts for a flood of mainly African migrants fleeing Libya and bound for Europe on unseaworthy boats that run into trouble.
Four of the rescued migrants including a pregnant woman were being treated in hospital, Lamloum said, adding that 38 women and seven children had been on the boat.
"We don't for the moment know where we will house them," he said.
With Tunisia struggling to deal with the influx, the Red Crescent has taken charge of assisting many of the rescued migrants, often with charitable help from local residents.
In April and May, Tunisian vessels rescued more than 450 migrants trying to make the dangerous crossing.
Libya has a coastline of 1,770 kilometres and has long been a stepping stone for Africans seeking a better life in Europe.
People smugglers, who have operated in Libya for years, have increased their lucrative trade, feeding on the political divisions and lawlessness that has gripped the country since the end of its 2011 uprising.
On April 19, some 750 migrants were killed when their trawler sank between Libya and southern Italy, sparking global outrage and demands for action.
The number of migrants entering the EU illegally in 2014 almost tripled to 276,000, according to European Union border agency Frontex, nearly 220,000 of them arriving via the Mediterranean.
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