The lawmakers said they would put the question of secession from Ukraine to a referendum in Crimea on March 16, further escalating tensions over the ex-Soviet state.
"I would like to ask you whether one can have an open, fair and democratic referendum under Kalashnikov guns?" Tymoshenko told reporters in Dublin at a convention of the European People's Party (EPP) yesterday.
"Who will count ballots? Who will insure that the people's free will was reflected? That's why this so-called referendum is illegitimate."
Earlier, Tymoshenko addressed delegates at the EPP conference in the Irish capital. It is the first time she has left Ukraine since being released from prison last month, where she was serving a sentence on charges of abusing power.
"The Kremlin must understand that Ukraine is a state and not a territory," she said, delivering her speech in a wheelchair because of chronic back problems.
"Ukraine is a sovereign nation that is free to join Europe. It is not a colony that will be driven into a cage."
She challenged Yanukovych in a bitterly contested 2010 presidential election, losing in a run-off and then finding herself the target of a string of criminal investigations that she claimed were aimed at ending her political career.
She was first arrested in August 2011 and two months later was sentenced to seven years in prison on charges of abusing her power over a 2009 gas deal she signed with Russia when she was leader.
Vitali Klitschko, the former Ukrainian boxer turned politician, also addressed the EPP convention, telling reporters afterwards the future of Ukraine "will be decided in the next few days."
"It's absolutely important to solve this conflict without any drop of blood."
Earlier yesterday, he joined a small group of people protesting against Russian aggression in his country, during which he condemned the decision by lawmakers in Crimea to vote to join Russia as a "provocation".
"It's a huge provocation against Ukraine and it's unconstitutional," he said.
He added: "Ukraine has to be an independent county. What's happening regards Crimea is provocation, I'm more than sure.
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