The Indian wild card had three match points in the 12th game of the second set but could convert none and went on to lose seven games on the trot to bow out of the ATP 500 event.
He lost 6-2 6-7(2) 0-6 in one-hour and 51 minute contest.
Yuki, ranked 107, had made a resounding start by taking the first from the Czech inside 20 minutes and was leading 2-0 in the second but allowed his rival to comeback.
Yuki had lost to Rosol in India's Davis Cup tie against Czech Republic and had a good chance to not only avenge his defeat but also gain some valuable points to better his rankings.
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