New highly sensitive malaria-detecting assays may help beat disease

An international team of scientists has come up with new assays that can detect malaria parasites in human blood at very low levels, which may be helpful in the campaign to eradicate the disease.

The team, led by Ingrid Felger, from the Swiss Tropical and Public Health Institute in Basel, Switzerland, took advantage of genes that have multiple copies in the parasite genome to reveal parasites present at concentrations that are 10 times lower than the detection limit of current standard assays.

The researchers compared three methods to detect malaria parasites in 498 samples randomly selected from a malaria survey in Tanzania, that is, light microscopy, the current standard molecular assay and the new assays.

Parasites were detected in 25 percent of samples by light microscopy, in 50 percent by the standard assay, and in 58 percent by the new assays. Compared to the new assays, the current molecular standard assay failed to identify 16 percent of infections, and at least 40 percent of those contained parasite gametocytes, the parasite stage that is transmitted when mosquitoes bite an infected person.

The new assays detect only the most common malaria parasite, P. falciparum, and while they can use very small blood samples collected "in the field," the analysis itself needs to be done in a biomedical laboratory.

Nonetheless, because low-density infections without disease symptoms are expected to become increasingly common as countries improve malaria control, ultra-sensitive tools such as these will likely be critical for malaria surveillance and for monitoring the progress of malaria control and elimination programs.

The study appears in PLOS Medicine.