"This is now the most Tweeted debate ever," Nick Pacilio, spokesman of Twitter said.
Donald Trump accounted for 64 per cent of the conversation on this social media website, while Clinton accounted for 36 per cent, according to the Silicon Valley- based social media company.
At the end of the 90 minute debate, Clinton earned 25,000 new followers, while Trump got 16,000 new followers.
"I'm a Muslim, and I would like to report a crazy man threatening a woman on a stage in Missouri," by Moustafa Bayoumi, author of The Muslim American Life and How does it Feel, became the most retweeted tweet from the debate.
This was followed by trying saying that he is a gentleman and Trump saying that Clinton, 68, would be in jail in his administration.
Pence congratulated Trump for winning the debate.
"Congrats to my running mate on a big debate win," he tweeted.
The former Deputy Secretary of State Nicholas Burns said that Clinton won the debate.
"Hillary Clinton won this debate on judgment, intelligence, maturity. Trump showed how disastrously ill- suited he is for the Presidency," Burns tweeted.
"Today was the most Tweeted day of the entire 2016 election, with nearly 30 million Tweets sent," the spokesman said.
The number of tweets during the first presidential face-off between Clinton and Trump exceeded the 10.3 million messages, Twitter had said.
Meanwhile, Trump also defended his early morning tweets in the recent week.
"Tweeting happens to be a modern day form of communication. You can like it or not like it. I have, between Facebook and Twitter, I have almost 25 million people. It's a very effective way of communication. I'm not un-proud of it, to be honest with you," Trump said.
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