The medical charity, also known as MSF, said it is "extremely concerned" because the fighting threatens its only cross-border supply routes between the two countries.
Scattered clashes have erupted in recent weeks as Iraqi forces have retaken disputed territory from the Kurds, part of a crisis sparked by last month's Kurdish vote for independence.
Federal forces have sought to regain control of the country's borders from forces loyal to the Kurdish regional government.
US officials say the fighting has also hindered the movement of military equipment and supplies in Syria and Iraq, where Iraqi, Kurdish and other allied forces are battling the Islamic State group.
A Kurdish official on the Syrian side of the border said access for aid workers and journalists has not been affected. The official spoke on condition of anonymity because he was not authorised to brief reporters.
"If the border between Syria and Iraq closes for humanitarian aid, the millions of already vulnerable people will no longer have access to critical medical care.
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