Musicians have a strong fan following on the microblogging website as among the 10 most followed people, seven are musicians.
The next most-followed musicians behind Bieber are Taylor Swift (85.5 million), Rihanna (76.9 mil) and Lady Gaga (69.5 mil).
Twitter has introduced a #100MBeliebers emoji to celebrate the 23-year-old musician's milestone number.
Bieber's most tweeted songs of all time are "Boyfriend", "Baby", "Sorry", "What Do You Mean" and "Love Yourself".
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