The denial follows reports of mounting tension between the two men, including the allegation that Andrew had said any husbands of Eugenie and Beatrice should be awarded the aristocratic titles of earls.
"There is no truth to the story that there could be a split between the Prince of Wales and I over my daughters' participation as members of the Royal Family," Andrew said yesterday in a statement on Twitter.
Andrew, the Duke of York, said his daughters' royal engagements were "very much appreciated by my family" and that he wanted them to be "modern, working, young women, who happen to be members of the Royal Family".
The two princesses have been heavily criticised by British tabloids for enjoying regular holidays.
Eugenie took eight holidays, including to Myanmar, over 15 months, ending with a New Year's party in New York last year, the Daily Mail reported earlier.
Prince Andrew's statement came a month after Prince Harry blasted "abuse and harassment" by the media against his girlfriend, US actress Meghan Markle.
Harry also took issue with the "outright racism and sexism" on social media directed against Markle, best known for her role as Rachel Zane in the US television drama "Suits".
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